Generations is designed to investigate identity stress, health outcomes, healthcare, and health service utilisation across three generations of lesbian, gay, or bisexual (LGB) adults in the United States of America. Adults were recruited from all fifty states through an existing survey. Eligible adults were educated to at least a sixth grade level, self-identified as Black, Latinx, or White, and self-identified as lesbian, gay, bisexual, queer, or same-gender loving. Only cis-gender and gender queer participants were included in the study. Transgender participants were recruited into a separate sister study. Participants were sorted into three generational cohorts. Cohort 1 consisted of adults aged between 18 and 25 years, cohort 2 with adults aged between 34 and 41 years, and cohort 3 included adults aged between 52 and 59 years. At baseline, all three cohorts amounted to 1,518 participants.